FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004982555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31938401
Sequences flagged as poor quality0
Sequence length125
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCG6900.031.9122816
TCCGCGC11050.019.392934
CGCGGGC3452.8805516E-818.9791493
CGCCGCG2851.8508235E-618.7975311
CCGCGCC13750.017.749155
TTCCGCG13300.017.007293
CCGCGAC4201.4693796E-817.007291
ACGCGGC5054.802132E-1016.5021232
CCTACGC22950.015.8175597
CGCGGGT6453.6379788E-1215.6888961
CGCGGCG3056.9375936E-515.613251
CGGTACG2350.003451970615.1920089
CGACCAT35600.015.0485861
GGCGCGA18550.014.91471530-31
GGTGCGC27650.014.6389575
CGCGACA4951.1070006E-714.4304291
AAGCGCG9950.014.352998
CGCACCG5402.0636435E-814.3302161
AGGCGCG16750.014.20859430-31
GCGCGAT17000.013.99964432-33
Produced by FastQC (version 0.11.5)