FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004982562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33622912
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1638700.4873759893253743No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA539890.16057205277163383No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC470750.14000869407147126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG429650.047.8630145
ACCGCGT432150.047.6274226
AGACCGC439600.046.8608634
CCGCGTT461100.044.650057
CGCGTTC471300.043.6837238
GCGTTCT530550.038.8501249
CAGACCG547500.036.9847073
CGGGGTC145900.036.3301352
TCAGACC581850.035.2636072
CTCAGAC603950.034.653081
CGCTACC383300.030.64327118-119
ACGGGGT130650.028.8850961
GCGGGTT31200.024.8017731
GTCTCGC225650.024.5431236
CTCGCTA229750.023.7167668
ACGCTAC472100.023.134253116-117
TACGGAG519950.022.92950834-35
GCCGCGT8850.022.8680741
GACGGGG97850.022.386231
GCTACCT466200.022.323303118-119
Produced by FastQC (version 0.11.5)