FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004982563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33622912
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA562950.16743047122152893No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA450340.13393842865246175No Hit
CTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTC403670.12005801282173299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT61400.047.294981
TGGCGCG47900.030.3123682
CGCGTGC99250.029.6789153
GCGCGTG101550.029.1819952
TCGTAGC43250.023.1157577
CGTAGCC41100.022.2978948
TATCGTA46650.020.4104825
CGCCGTA24950.020.3892746-47
TAAGATC289250.020.2230072
GCGTGCC153050.019.4411334
CGACCTA40850.019.2289333
TCTATAG407300.019.037213
GTGGCGC82400.018.9929391
AGGGATC205850.018.8478722
CTAAGAT332450.018.4005321
CGCGATT34000.017.58685332-33
ATCGTAG57650.017.341836
GTCGCCG32450.017.3273344-45
GTATCGT58900.017.1758634
CTATAGT464250.016.8561654
Produced by FastQC (version 0.11.5)