FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13726116
Sequences flagged as poor quality0
Sequence length55
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1302450.9488845934275946No Hit
TTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCG837390.610070612837601No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA788160.5742046766907696No Hit
GTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCC663770.48358180857571065No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAG529080.3854549968833135No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTC386770.28177672402010884No Hit
CGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTT197190.14366044990440122No Hit
GGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAAT157560.11478848058693371No Hit
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCC147420.10740110312341816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATAG115200.025.2172243
CAATAGA115550.024.9713484
TTCAATA117100.024.8498972
ATAGATC123500.023.304386
TTTCAAT126500.023.2044091
AGATCGC125000.022.9463468
ATCGCAG126750.022.5911310
TAGATCG127750.022.4715677
AATAGAT128900.022.4611115
TCGCAGC128400.022.35797311
GATCGCA134550.021.4633149
GCAGCGA134950.021.29102313
GTTTAGC194400.019.5398851
AGCGAGG150500.019.0423615
CTCGTAT3800.018.69853845
TATCTCG3750.018.29461142
CGCAGCG158300.018.2588112
CGAGGGA156550.018.24366817
TTTAGCG283550.016.5271471
TAGCGCC286600.016.3494783
Produced by FastQC (version 0.11.5)