FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20081955
Sequences flagged as poor quality0
Sequence length55
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2296851.1437382465999948No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1350450.6724693885630159No Hit
TTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCG1012430.5041491229315074No Hit
GTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCC666700.3319895896589749No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTC540210.2690026942098018No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAG342450.1705262261567661No Hit
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCC316430.15756932031766827No Hit
CGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTT240510.11976423610151501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT9550.023.09015845
TATCTCG9900.022.52132642
TCAATAG87150.022.259743
AGATCGC87350.021.9285268
ATCGCAG88350.021.65297710
TTCAATA90650.021.4813562
TCGCAGC89300.021.39503911
CAATAGA92300.021.1771134
CGTATGC11350.020.72319247
TCGTATG11000.020.4918846
TATGCCG11650.020.39843449
ATAGATC94700.020.35596
GCAGCGA95950.019.9632713
GATCGCA97400.019.9425099
TTTAGCG308050.018.5493341
GTTTAGC200000.018.5034541
AATAGAT106150.018.4140135
TAGCGCC310950.018.3853823
TTAGCGC313350.018.2523822
AGCGAGG108750.017.74871315
Produced by FastQC (version 0.11.5)