FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23925009
Sequences flagged as poor quality0
Sequence length55
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCG1037580.43368008764385413No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTC707430.2956864091461784No Hit
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCAGCCGCGCCC624930.2612036634970545No Hit
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCC589650.24645758753946548No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAG582860.24361955307937397No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT547540.22885675821480359No Hit
GTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCC451930.188894390802528No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA361810.15122669337344868No Hit
GGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAAT293230.12256212735384968No Hit
CTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGC292680.12233224238285553No Hit
CGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGC280000.11703234886975382No Hit
CGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTT279510.11682754225923175No Hit
CCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCC255680.10686725342506664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATAGA122000.025.4215894
TCAATAG122250.025.409713
TTCAATA125000.024.9476182
AATAGAT135300.022.9714325
TTTCAAT137450.022.851871
AGATCGC138150.022.445678
ATAGATC138550.022.3822236
ATCGCAG145750.021.25725610
TCGCAGC147300.021.05019611
GATCGCA150600.020.8338419
GCAGCGA159000.019.48581713
TAGATCG170750.018.1315987
TAGCGCC349150.016.5789073
AGCGAGG187950.016.35413215
TTTAGCG366650.015.76812651
TTAGCGC370500.015.6095982
CGAGGGA203350.015.10356517
TCTCCGA29600.015.0601537
TTTCCGG221700.014.99951641
CTTTCCG224900.014.81878340
Produced by FastQC (version 0.11.5)