FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13430481
Sequences flagged as poor quality0
Sequence length55
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT888970.6619048118976528No Hit
GTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCC648570.4829089888887822No Hit
TTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCG533380.39714139798864984No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390030.2904065759074452No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTC292100.2174903490053707No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAG234760.17479642017288882No Hit
TGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGTC207830.15474501620604653No Hit
CGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTT168920.12577360408759747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG5600.024.06155842
CTCGTAT5550.023.39530445
GTTTAGC168000.022.8991951
TATGCCG6550.020.20430249
ATAGATC69950.018.7012336
TAGATCG69850.018.6578667
CAATAGA72050.018.5702484
CCGACTA22500.018.18332716
TCAATAG73000.018.1271673
TTCAATA74450.018.0703542
TCGTATG7250.017.90944346
AGATCGC73000.017.785658
AATGCCG21550.017.73361612
CGACTAT23100.017.71188717
GATCGCA75150.017.6353879
TATCGTA6000.017.556345
CGTATGC7150.017.47535947
AATAGAT76800.017.25655
TTTAGCG229350.016.7748992
ATCGCAG78600.016.64314710
Produced by FastQC (version 0.11.5)