FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13260378
Sequences flagged as poor quality0
Sequence length55
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT358230.27015066991302966No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA245080.1848212773421693No Hit
TTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCG219620.16562122135583165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT7950.032.35692245
TATGCCG8450.030.44070449
TATCTCG8650.030.02223242
CGCCAAT8700.029.85430534
TCGTATG8750.029.6784546
CGTATGC8800.029.50926647
ACGCCAA9050.028.6997233
ATCGCAG26600.023.85293810
AGTCACG11100.022.95479429
TCGCAGC27950.022.8759511
CAATAGA30050.022.0128254
AGATCGC30400.021.4377548
GCAGCGA30400.021.35466413
ATAGATC30300.021.1843516
TCAATAG30700.021.1475813
GTATGCC12650.021.10853648
TTCAATA34750.019.669922
TAGATCG32850.019.316827
CGTCTGA13850.019.1021117
GTATCTC14500.018.24773641
Produced by FastQC (version 0.11.5)