FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19324654
Sequences flagged as poor quality0
Sequence length55
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1331900.6892232067906623No Hit
GTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCC1278760.6617246549407819No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAG955910.49465827434737No Hit
TTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCG928350.4803966994700138No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750200.3882087617196147No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTC540830.2798652953889886No Hit
CGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTT334520.173105298547648No Hit
TGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGTC297360.15387597625292543No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGC271930.14071662033379742No Hit
GGCGAAGCTACCATCTGTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGC262700.13594033818147533No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC245190.12687937388167467No Hit
GGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAAT239820.12410054017008533No Hit
GCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGG232600.12036438013327432No Hit
TTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGA229460.11873951274884403No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAG214220.11085321372377482No Hit
GGAACCGCAGGTTCAGACATTTGGTGTATGTGCTTGGCTGAGGAGCCAATGGGGC206020.10660992947144098No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACG205400.1062890957840694No Hit
ATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTC199970.10347921365112152No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGT199380.1031739041744292No Hit
CAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCT196290.10157491047446437No Hit
CGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAG196020.10143519257835094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAATA232700.023.5058562
TCAATAG232500.023.367973
CAATAGA234650.023.122534
TTTCAAT242100.022.423471
TATCGTA8100.022.077785
AATAGAT247400.021.9920855
ATAGATC247350.021.8578766
GTTTAGC333600.021.6166781
AGATCGC253650.021.199098
TAGATCG254850.021.1088817
GATCGCA263000.020.5385769
ATCGCAG277700.019.4337310
TCGCAGC281100.019.18124211
AGTATCG10000.019.1149373
GCAGCGA287500.018.75430113
ATCGTAG10400.018.1373966
AATTGCC40450.018.0474285
AATGCCG38400.017.60745212
AAATTGC42150.017.5003664
AGCGAGG314700.017.03258115
Produced by FastQC (version 0.11.5)