FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37161622
Sequences flagged as poor quality0
Sequence length55
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCG2396520.6448911191228414No Hit
GTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCC1904150.5123969023741751No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTC1756870.47276461721719254No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAG1212700.32633128876882717No Hit
CGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTT1026760.27629579785295705No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT641180.1725382169809488No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGC632920.1703154937639697No Hit
TGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGTC597360.16074648194850052No Hit
GGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAAT581470.15647056525143063No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC576740.15519774675066658No Hit
GGCGAAGCTACCATCTGTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGC544070.14640641896631962No Hit
GCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGG534310.14378005351865428No Hit
CGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAG487390.13115412454278771No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGT474430.12766665566965835No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTATGACT467630.12583681089054724No Hit
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCC452500.12176540625702505No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACG430820.11593143054950616No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAG408310.10987410614100752No Hit
CAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCT405200.10903722124938466No Hit
GAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACC400130.10767291050966506No Hit
GCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTT384700.10352077743000562No Hit
CCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCC375070.10092939430899975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAGC406500.024.7087631
TTCAATA272400.023.1308542
TCAATAG278500.022.6154143
CAATAGA281500.022.4701654
TTTCAAT283050.022.186971
ATAGATC290350.021.7002436
AATAGAT295350.021.4324115
ACTTTCA122100.021.3360614
AGATCGC298650.021.0069278
TAGATCG301200.020.8290797
TGGCGAC22400.020.5807611
TGACTTT127900.020.0619352
TTGTCTC76900.019.8863412
GATCGCA321850.019.7895229
TTTAGCG666700.019.7549671
TTAGCGC686250.019.2737712
TAGCGCC690550.019.1537553
CTTGTCT80800.019.0893381
ATCGCAG339650.018.49997710
ATCGTAG8400.018.372866
Produced by FastQC (version 0.11.5)