FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24362947
Sequences flagged as poor quality0
Sequence length55
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2029680.8331011843517946No Hit
TTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCG1441940.5918577912598176No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1055990.4334409954592111No Hit
GTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCC904080.3710881117953423No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTC788570.32367594938329913No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAG559720.22974232140307166No Hit
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCC473780.1944674427112615No Hit
CGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTT341820.1403032235796433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATAG120400.023.969513
CAATAGA124100.023.2549114
TTCAATA126600.023.1052632
ATAGATC125950.022.7188266
AGATCGC128150.022.2724578
ATCGCAG129600.022.2102110
TCGCAGC133750.021.5027111
AATAGAT135950.021.3180145
TAGATCG137250.020.777897
GCAGCGA141950.020.2781613
GATCGCA142450.020.223899
CTCGTAT7800.020.10128845
TTTAGCG434700.019.5300581
TAGCGCC434800.019.376843
TTAGCGC440500.019.254022
TTTCAAT158050.018.5000671
TCGTATG8700.018.0217746
GTTTAGC274150.017.6803251
CGTATGC9300.017.38549647
AGCGAGG165150.017.22110415
Produced by FastQC (version 0.11.5)