FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20095087
Sequences flagged as poor quality0
Sequence length55
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCAGCCGCGCCC910560.4531256819141912No Hit
TTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCG904070.4498960367775467No Hit
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCC748140.3722999557055911No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTC732910.3647209887670554No Hit
CGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCAGCCGCGCCCCG321090.1597853246417893No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAG290690.14465724880912434No Hit
CGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTT281860.1402631399406233No Hit
CCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCC271670.13519224873223987No Hit
CGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCCCG271140.13492850267331513No Hit
CGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGC270140.1344308685998722No Hit
GTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCC265790.13226616038039546No Hit
CTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGC254450.12662298998755267No Hit
GGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAAT227060.11299279271595093No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207090.10305504026929567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAATA73450.023.3983822
TCAATAG73100.023.2428593
CAATAGA73850.023.0068114
ATAGATC74650.022.3791376
AGATCGC76600.021.7756128
AATAGAT77850.021.7256765
ATCGCAG78700.021.31820310
TCGCAGC81300.020.69669211
TTTCAAT84650.020.6105561
TAGATCG83550.019.876297
GATCGCA85850.019.6289449
GCAGCGA92100.018.24312613
TAGCGCC302400.017.0745893
ACGAAAT18200.016.9598747
TTTAGCG318650.016.2331851
TTAGCGC322000.016.0575892
AGCGCCA346900.015.0963054
CGCAGCG112850.014.93212512
AGCGAGG113350.014.86629615
CCGCGCC633550.014.80179548
Produced by FastQC (version 0.11.5)