FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23761916
Sequences flagged as poor quality0
Sequence length55
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCC1507110.6342544094508203No Hit
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCAGCCGCGCCC1506950.6341870748133274No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTC1291130.5433610656649068No Hit
TTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCG890510.3747635502120284No Hit
CGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCCCG687450.28930747840367754No Hit
CGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCAGCCGCGCCCCG683870.28780086588977083No Hit
CTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGC448520.18875582255235648No Hit
CCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCC432540.1820307756327394No Hit
GGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAAT424140.17849570716435495No Hit
CGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCC397080.16710773659834502No Hit
CGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTT383110.1612285810622342No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAG319270.1343620607025124No Hit
CGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGC311550.13111316444347335No Hit
TTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGG266700.11223842387120635No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAG258870.10894323504889084No Hit
CCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAA255370.10747028985373065No Hit
GAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCA252580.10629614211244581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAATA54700.025.67552
TCAATAG55350.024.9311583
CAATAGA59100.023.4736484
ATAGATC58550.023.3869826
AGATCGC60600.022.554618
AATAGAT62900.022.0919365
ACGAAAT43000.021.76131447
TTTCAAT65700.021.3990151
ATCGCAG65900.020.85216910
TCGCAGC68250.020.2418511
CGAAATT46900.019.95194648
TAGATCG68550.019.9035557
GCAGCGA77700.017.52791613
GGCACGA54600.017.36238944
GCTTCTA57650.016.878854
ATCGTGT58300.016.34386624
AATCGTG58350.016.3298623
CACGAAC1031800.016.2249871
CGTGTGA58800.016.07991426
TGACCGC58700.016.02590830
Produced by FastQC (version 0.11.5)