FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26562721
Sequences flagged as poor quality0
Sequence length55
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCG1857890.6994351218762566No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTC1422420.5354948388005882No Hit
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCAGCCGCGCCC1160350.43683401259983873No Hit
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCC1016520.3826866983996105No Hit
GTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCC731270.2752993565681769No Hit
CGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTT559670.21069754111410502No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT532980.20064962471276945No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAG516680.19451320517954465No Hit
CGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCAGCCGCGCCCCG474630.17868274865364886No Hit
CCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCC437630.16475345278068465No Hit
GGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAAT411040.1547431831249517No Hit
CGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCCCG407760.15350836986918623No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA365250.1375047383135184No Hit
CTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGC311370.11722067178283431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAATA114850.024.6270352
TCAATAG114750.024.4562643
CAATAGA115900.024.382784
ATAGATC118350.023.6987326
TAGATCG124000.022.539817
AGATCGC124500.022.4880058
AATAGAT125950.022.4727235
TTTCAAT131850.021.7361221
ATCGCAG131200.021.39561510
GATCGCA137150.020.5388479
TCGCAGC139850.020.10728511
TAGCGCC543700.019.3841363
TTTAGCG559950.018.877561
TTAGCGC567850.018.594282
AGCGCCA588800.018.2240664
GCAGCGA154900.018.0748113
ACGAAAT30550.017.47973447
CGCAGCG166350.016.8305812
CGCCAGG662750.016.0297816
CGAAATT33900.015.68024548
Produced by FastQC (version 0.11.5)