FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13356556
Sequences flagged as poor quality0
Sequence length55
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCG608130.4553044961590398No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT472910.35406582355511407No Hit
GTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCC467020.3496560041375935No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTC319710.23936559693980994No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAG288340.21587900353953518No Hit
TGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGTC259510.1942940979695664No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA208760.15629777616325646No Hit
CGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTT179790.134608053153822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4400.027.83931545
GTTTAGC121850.022.5793761
ATAGATC85450.020.4404776
TAGATCG85150.020.4261867
AGTCACG6600.020.41572829
CAATAGA86650.020.3639934
TCAATAG86650.020.3074253
TTCAATA88100.020.1400992
AGATCGC88300.019.7807378
TCGTATG6550.019.07510246
AATAGAT92600.019.0738285
TTTCAAT93850.019.0213681
GATCGCA94000.018.9982349
TTTAGCG191050.018.983041
CGTATGC6800.018.73485447
TTAGCGC197100.018.489422
ATCGCAG95350.018.39525810
TAGCGCC199000.018.312893
TCGCAGC97250.017.93510811
TATGCCG6900.017.75912349
Produced by FastQC (version 0.11.5)