FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23945222
Sequences flagged as poor quality0
Sequence length55
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2027510.8467284203921769No Hit
TTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCG1731370.7230544782587525No Hit
GTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCC1140380.47624532359733396No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA968430.4044355905324244No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTC769080.3211830736002364No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAG724170.30242776617397826No Hit
CGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTT359540.15015104057084958No Hit
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCC354500.14804623653102902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATAG164200.024.1987633
CAATAGA168050.023.673434
TTCAATA168700.023.6694412
AGATCGC172850.022.775148
ATCGCAG176100.022.25832210
ATAGATC177800.022.2374736
TCGCAGC177200.022.13416111
GATCGCA184600.021.3788269
AATAGAT188400.021.1033445
GCAGCGA185650.021.10071213
TAGATCG195700.020.0658387
TTTCAAT208100.019.3318521
AGCGAGG205100.018.92087715
CGCAGCG209500.018.72178612
TTTAGCG533850.018.4947431
GTTTAGC343500.018.338761
TAGCGCC543150.018.117473
TTAGCGC544550.018.102382
CGAGGGA214450.018.08420417
AGCGCCA596850.016.6350864
Produced by FastQC (version 0.11.5)