FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20496817
Sequences flagged as poor quality0
Sequence length55
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1504520.734026166111548No Hit
TTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCG1159850.5658683492173443No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA949520.4632524162166253No Hit
GTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCC712400.34756616112638367No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTC582280.28408313349336145No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAG409650.19986030026027946No Hit
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCC342690.16719181324592985No Hit
CGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTT250290.12211164299315352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATAG98100.022.5493223
CAATAGA101500.021.7456514
TTCAATA103650.021.5073452
AGATCGC102300.021.3600838
ATCGCAG102500.021.3431910
ATAGATC102800.021.2323596
TCGCAGC104800.020.78132411
TAGATCG110700.019.761397
AATAGAT113300.019.5889855
GATCGCA113000.019.4677529
TTTAGCG339400.019.3704571
GCAGCGA113500.019.27496713
TTAGCGC346300.018.979422
TAGCGCC346850.018.942263
GTTTAGC215600.018.7396621
CTCGTAT6900.017.40061245
AGCGAGG129350.016.83770615
AGCGCCA398650.016.6959674
CGAGGGA132600.016.42461417
TTTCAAT137450.016.2053341
Produced by FastQC (version 0.11.5)