FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19486596
Sequences flagged as poor quality0
Sequence length55
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1589080.8154733643577359No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA934850.47974002232098417No Hit
TTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCG585350.3003859678724801No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTC364660.18713376107350918No Hit
GTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCC268590.13783320596373017No Hit
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCC204200.104789979737867No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAG203790.10457957870117489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCC157300.021.5224693
TTTAGCG158650.021.4011781
TTAGCGC160100.021.1613622
ATCGCAG58850.019.648110
AGCGCCA176150.019.3027174
TCGCAGC60200.019.12624711
CTCGTAT9500.018.82864245
TAGATCG62800.018.5677367
AGATCGC65050.017.8878448
CGCAGCG65300.017.82031812
GATCGCA66550.017.5584169
TATGCCG10500.017.49987249
GCGCCAG199900.017.266725
ATAGATC68850.017.114056
GCAGCGA70100.016.56532113
TCAATAG76500.015.9790843
CGCCAGG217750.015.8175366
AGCGAGG76150.015.31373115
CGTATGC11650.015.14253147
CGAGGGA76350.015.04896517
Produced by FastQC (version 0.11.5)