FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004982638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21712905
Sequences flagged as poor quality0
Sequence length55
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2585651.190835588328692No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGGTATCTCGTATGCCG2119800.9762857618545284TruSeq Adapter, Index 5 (97% over 41bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1373900.6327573394716184No Hit
TTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCG905070.4168350573080848No Hit
GTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCC864370.39809044436937385No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAG660920.3043904074558425No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTC496840.22882244453241055No Hit
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCC240780.11089257747869297No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)