FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17597287
Sequences flagged as poor quality0
Sequence length55
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1173970.6671312458562504No Hit
GTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCC537800.30561529172082036No Hit
TTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCG434530.24693010916966915No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA411200.2336723837032379No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAG263820.14992083722905697No Hit
TGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGTC208570.11852395201601247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT23450.038.96701445
TATCTCG23800.038.49580842
TCGTATG25650.035.81562846
TATGCCG25600.035.60213549
CGTATGC26400.034.7053447
CGTCTGA32600.028.47970617
CACTATC31950.028.29301339
GTATGCC33100.027.7547248
ACACGTC34650.026.72405614
GACCACT35800.025.38747636
CTATCTC39300.023.81165541
ACTATCT40350.022.9492440
CACACGT41000.022.76420413
CACGTCT42850.021.72434215
ACGTCTG46400.021.3293316
CAGTCAC44650.021.12329928
CCACTAT44500.020.80926538
CGACTAT48150.020.65587417
AGTCACT46250.020.23364429
CCGACTA49150.020.13593116
Produced by FastQC (version 0.11.5)