FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17788545
Sequences flagged as poor quality0
Sequence length55
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCC1122380.6309566071873782No Hit
TTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCG715460.4022026534491719No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT567780.31918293486060834No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAG560330.3149948464025585No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTC377530.21223208531108081No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGC278770.15671321066450347No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260820.14662244719846396No Hit
CGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTT256870.1444019170764107No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC238250.13393450672890897No Hit
GGCGAAGCTACCATCTGTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGC234990.1321018666788093No Hit
TGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGTC228470.12843658657860998No Hit
GCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGG218180.12265196507078009No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGT205300.11541135039431275No Hit
CGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAG198640.11166736796067359No Hit
GAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACC189150.10633247407250003No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTATGACT182990.10286957140114607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAGC265250.023.5542771
CTCGTAT4800.022.96729745
TCAATAG145550.020.9276083
CAATAGA147250.020.669364
TTCAATA148700.020.6655642
ACGTTAT5500.020.48984138
CGACTAT15350.020.11043717
ATAGATC150500.020.0698646
TTTCAAT154150.019.788031
TATCTCG5750.019.5989842
TAGATCG154100.019.4579327
AATAGAT157350.019.3985425
AGATCGC155200.019.3200198
CAAGTCG17100.018.48144128
GATCGCA164000.018.477529
CCGACTA16850.018.1741816
TTTAGCG343250.018.1478732
TTAGCGC349250.017.8360963
ATCGCAG169350.017.79253210
TAGCGCC350550.017.7489814
Produced by FastQC (version 0.11.5)