FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21266076
Sequences flagged as poor quality0
Sequence length55
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCAGCCGCGCCC739300.3476428843760363No Hit
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCC620330.2916993243135217No Hit
TTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCG593400.2790359631932097No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTC521750.24534380484674276No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT483650.22742794674485317No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA332510.15635700728239663No Hit
CCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCC224930.10576939535060441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCAG54200.018.21042610
AGATCGC54050.018.1711948
TCAATAG58100.017.4700933
TCGCAGC57050.017.42949111
TAGCGCC207700.017.2458553
ATAGATC57650.017.1224966
TTCAATA59650.016.8512122
CGCGATT22100.016.5441881
TTTAGCG222400.016.1752361
TTTCCGG216050.016.08440241
TTAGCGC224300.016.0018252
GATCGCA62500.015.9101279
CTTTCCG218000.015.89556140
CCGCGCC481150.015.73769448
CAATAGA65250.015.5933194
TTCCGGC222100.015.58995642
GCAGCGA65250.015.126513513
AGCCGCG276400.015.08563246
TCCGGCC231700.015.0044443
CACGAAC513750.014.7781441
Produced by FastQC (version 0.11.5)