FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12790804
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC47650.0110.651251
CGATTGA26750.0109.591981
TAAACCG32900.0109.466771
CACCGAA31950.0107.49028
ATGCGAT41650.0106.7034151
CTCTACG42400.0104.136451
GATCTAG54900.0102.730071
TACACGA41300.0100.457091
CGGACGT40100.0100.050441
TCGCGAG39300.099.704441
ATCCGAA54800.099.237211
GGCTCGA40750.097.924261
GCATGCG47850.097.393621
ATGCGAG19700.097.2195743
TAAGCGT51100.097.119511
TCCCGAT45300.096.992861
TAGCCGG41700.096.038831
GATCGTA53000.094.996911
TTGAACG30850.094.79361
GCCTCTA75550.093.3375551
Produced by FastQC (version 0.11.5)