FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12790804
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC6593305.154718968408866No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC4423983.458719248610173No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC4294483.3574746356835736No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC2796322.186195644933657No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC613080.4793131065099583No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT569380.4451479359702486No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC414960.32442057590750356No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT379880.2969946220737962No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG263910.20632792121589855No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG207010.16184283646282124No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG192520.15051438517860177No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA188650.14748877396604623No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG160640.12559022872995318No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT153340.11988300344528773No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA153130.11971882299189325No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG134750.10534912426146158No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG862300.0123.856391
AGTCTCT645150.0101.8790051
GTCTCTC674600.097.4634552
TCTCTCA716450.091.834583
TCAGCTG775500.084.691687
CAGCTGG810050.081.1584858
CTGATGG2144350.077.189291
GGCTCAA2234950.074.219496
CTCAAAC2240000.074.0594948
GCTCAAA2246450.073.857337
TCAAACA2265500.073.286289
ATGGCTC2317000.071.501094
GATGGCT2319500.071.433343
TGGCTCA2364050.070.050655
TGATGGC2380550.069.799782
AGCTGGT963300.068.112569
TCTCAGC1058500.062.403495
CTCAGCT1083200.061.179926
CTCTCAG1109400.059.5533524
TACGGCG16750.049.0039443
Produced by FastQC (version 0.11.5)