FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12931882
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAA32600.0115.064878
ACGATAC46750.0112.325531
CGATTGA29150.0105.763971
TAAACCG34850.0104.5876161
ATGCGAT39600.0103.320311
GATCTAG52950.0102.302081
ATCCGAA54600.0102.112961
TACACGA41150.0101.879241
GGCTCGA39000.0101.215841
CTCTACG45750.0100.137921
TCGCGAG38150.099.317041
TAAGCGT53900.099.162551
CGGACGT40150.098.496161
CCTTACG36300.098.028621
TAGCCGG43400.096.763461
ATGCGAG19850.096.482553
CGAACAT54000.096.0442351
CGCTATC27200.095.867681
GATCGTA51150.095.762581
TCCACGA32000.094.947848
Produced by FastQC (version 0.11.5)