FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12931882
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC6647895.140698005131813No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC4452453.4430023410358985No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC4323653.343403535541076No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC2829782.1882197811579163No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC615470.4759322734308897No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT573840.44374051665488445No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC420980.3255365305684045No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT385090.2977834162111903No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG263100.20345066557211086No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG204420.1580744395904633No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG195040.15082104832073168No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA187380.1448977032113346No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG163260.12624612566059604No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA156610.12110379602906986No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT154060.11913192526810869No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG136750.10574640257311348No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG860800.0123.461431
AGTCTCT650750.0101.438121
GTCTCTC676500.097.415462
TCTCTCA718450.091.837823
TCAGCTG778850.084.622357
CAGCTGG811550.081.398018
CTGATGG2165250.078.2683641
GGCTCAA2259850.075.076236
CTCAAAC2273650.074.672658
GCTCAAA2274800.074.619947
TCAAACA2298350.073.8832559
GATGGCT2345600.072.319333
ATGGCTC2347900.072.251554
TGGCTCA2386400.071.022555
TGATGGC2410600.070.560792
AGCTGGT972500.067.675349
TCTCAGC1063050.062.304585
CTCAGCT1085800.061.2312476
CTCTCAG1116250.059.3867764
TACGGCG16750.042.125433
Produced by FastQC (version 0.11.5)