FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10547635
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAA27350.0113.4609768
ACGATAC39550.0106.911421
ATGCGAT34300.0106.474671
GATCTAG44700.0106.035391
TAAGCGT43650.0104.9555361
TACACGA33250.0104.854291
TAAACCG27900.0104.04791
CGATTGA24700.0103.237811
TAACGCA17400.0100.986162
GGCTCGA31500.0100.617751
ATCCGAA44800.0100.010321
TAGCCGG37900.099.9721451
CTCTACG36550.099.723061
CCTTACG29100.099.5097351
TCGCGAG31850.099.285891
CGGACGT34600.098.681271
CGAACAT43300.097.985061
GATCGTA40950.096.747831
TACGGAT28750.095.7101441
ACCGAAT12500.095.614699
Produced by FastQC (version 0.11.5)