FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10547635
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC5071664.808338551722732No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC3522303.339421585976382No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC3245993.0774576480888842No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC2219712.104462279932895No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC460690.4367708969830678No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT427150.4049722994775606No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC327350.31035393242181775No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT297500.28205374948981454No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG205790.19510534826053422No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG167340.15865167878865735No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA149250.14150091466001619No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG140150.13287338820503364No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG136980.129867975143243No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA118240.11210095912496024No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT110700.10495243720511754No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG107970.10236417926862276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG675850.0123.3977051
AGTCTCT521150.099.020581
GTCTCTC534400.096.632822
TCTCTCA569750.090.7241743
TCAGCTG619050.083.322637
CAGCTGG647000.079.743898
CTGATGG1696750.077.426531
GGCTCAA1759550.074.737356
CTCAAAC1762150.074.554448
GCTCAAA1769850.074.2803047
TCAAACA1785850.073.649699
ATGGCTC1830150.071.6339654
GATGGCT1832050.071.579323
TGGCTCA1862800.070.335895
TGATGGC1895000.069.383362
AGCTGGT773050.066.59229
TCTCAGC853700.060.860385
CTCAGCT867850.060.083786
CTCTCAG888850.058.3645254
AGGCAGT59800.041.7868582
Produced by FastQC (version 0.11.5)