FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10633175
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC5117684.812936869749628No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC3566853.3544543374862164No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC3282083.0866415722491167No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC2238432.105137929169792No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC464700.43702845105060345No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT435050.4091440233044223No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC330270.31060337105333075No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT304150.286038741956189No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG209360.19689321392716663No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG167350.157384788644972No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA152040.14298645512746663No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG141440.13301765465159748No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG137970.12975428317506296No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA119130.11203615100851816No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT113660.10689187378181964No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG107360.10096702066880307No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG678700.0123.567681
AGTCTCT519950.0100.032051
GTCTCTC539850.096.1965562
TCTCTCA574450.090.531213
TCAGCTG628650.082.725937
CAGCTGG654200.079.6700068
CTGATGG1710900.077.1496051
GGCTCAA1770000.074.717726
CTCAAAC1773800.074.6455468
GCTCAAA1780650.074.343277
TCAAACA1799250.073.741769
ATGGCTC1839250.071.7244344
GATGGCT1846800.071.454273
TGGCTCA1870850.070.4860155
TGATGGC1904150.069.504122
AGCTGGT784600.066.181149
TCTCAGC867000.060.266095
CTCAGCT877700.059.990786
CTCTCAG908350.057.5147254
AGGCAGT55550.036.559662
Produced by FastQC (version 0.11.5)