FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9710002
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAA20600.0111.841718
ACGATAC35950.0110.000221
CGATTGA21500.0105.198921
ATGCGAT32100.0105.017331
TAAACCG23900.0104.279361
TAAGCGT39050.0103.665781
TACACGA33000.0102.371491
ATCCGAA41150.0100.475951
GATCGTA37800.0100.2337651
GATCTAG41750.0100.067171
TCCACGA23800.099.526798
CTCTACG33400.099.420191
CGCTATC21050.098.893071
ATGCGAG14350.097.838143
TCGCGAG30000.096.7617951
GGCTCGA31450.096.42321
TCGACTA11950.096.399139
CCTTACG28250.096.3814541
CCGGTAG31450.095.965131
TCCCGAT33500.095.898131
Produced by FastQC (version 0.11.5)