FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9815413
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC4791224.8813228745443515No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC3292783.3547034648465632No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC3118193.176830154778001No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC2086932.126176453298501No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC443300.4516366249693212No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT414200.42198937528150876No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC315030.3209544010017714No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT284430.2897789425671645No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG196210.19989989213902667No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG152670.1555410862487396No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA142850.14553641298639192No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG138890.14150194189485454No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG126050.128420475022294No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT115320.1174886884535577No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA113930.11607254834819483No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG101410.1033170993416171No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG636300.0123.29961
AGTCTCT475150.0100.04361
GTCTCTC491100.096.850072
TCTCTCA524700.090.895313
TCAGCTG563800.084.233227
CAGCTGG590350.080.603118
CTGATGG1581350.076.594331
GGCTCAA1632200.074.104116
CTCAAAC1638400.073.888488
GCTCAAA1642150.073.69827
TCAAACA1660350.073.0591059
ATGGCTC1697800.071.262064
GATGGCT1706850.070.955933
TGGCTCA1728600.069.917355
TGATGGC1764250.068.794562
AGCTGGT710250.066.773189
TCTCAGC788050.060.6113475
CTCAGCT810500.059.2345166
CTCTCAG820900.058.282344
TACGGCG13350.044.2246933
Produced by FastQC (version 0.11.5)