FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8523058
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA25050.0121.3350141
TGACTAG36550.0110.746591
CGCTATC26100.0108.450971
CCGGTAG26900.0100.673921
GCGCAAC23550.0100.620481
ATGCGAT29250.099.972741
TAAACCG20000.099.754211
ACGATAC24550.099.749071
CTCGAAA25450.099.334641
TCCCGAT30900.099.0630951
ATCCGAA25850.098.633431
ATAGACC34700.098.592881
CGTTGGG33950.098.437291
TACACGA21350.097.494791
GATCTAG22200.096.032921
CGACCAA21650.095.457732
TATGCCC33050.095.451781
AGCGAAC20400.094.587939
CGTAACA14300.093.649493
CTAATGG33000.093.63211
Produced by FastQC (version 0.11.5)