FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9221419
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG37750.0109.715051
CGTAACA20700.0109.56443
TCGTACC41250.0109.486081
CGAACAT42850.0108.08751
CGATTGA17750.0107.5382161
ACGATAC33100.0105.978141
AACGTTG38150.0104.03331
ACCGTAA47550.0103.917561
CACCGAA17050.0103.4579548
CGGACGT26950.0102.633181
ATCCGAA34200.0102.569481
GGCTCGA27050.0102.5200351
CGTAAAG18250.0102.180063
ATTACGA11700.0102.151429
TATTACC47400.0102.118921
TCCCGAT33800.0102.078451
TCGACCA19250.0101.732648
ACGGGTC44950.099.352151
ATGCGAT21900.099.329471
TGACGGC29950.099.086771
Produced by FastQC (version 0.11.5)