FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9221419
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC5702386.183842204762629No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC3799004.119756406253745No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC3499893.795392010708981No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC2286902.4799870822484045No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC502510.5449378235605605No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT449780.4877557347735744No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT338040.3665813254988196No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG331060.3590119915383955No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC314640.3412056213908076No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT278210.301699770935471No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG227780.24701187528730661No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA227070.2462419287096704No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG221840.24057035039834976No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG201460.21846963032479058No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG158570.17195835044476343No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAG130560.14158341574111316No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGGAGA130150.14113879870332321No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG114580.12425419558529983No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA112650.12216124221228859No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTA104760.11360507531433069No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGA104090.11287850600867394No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG736100.0124.5758061
AGTCTCT533600.0112.075661
GTCTCTC589000.0101.485172
TCTCTCA650400.092.161953
TCAGCTG689700.086.656137
CTGATGG1721300.080.117861
CAGCTGG748400.080.070998
GGCTCAA1841900.075.0204246
TGATGGC1843050.074.973842
CTCAAAC1852750.074.6788948
GCTCAAA1851300.074.667387
TCAAACA1873950.073.949329
GATGGCT1878050.073.49973
ATGGCTC1883800.073.286824
TGGCTCA1932300.071.421265
AGCTGGT848000.070.5051049
TCTCAGC878800.068.16815
CTCAGCT930400.064.8905266
CTCTCAG930300.064.556954
TACGGCG10650.048.6777843
Produced by FastQC (version 0.11.5)