FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004982881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9322139
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC5755056.173529487170272No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC3834984.113841254673418No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC3532423.789280550311468No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC2314872.4831961849099224No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC503590.5402086366658982No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT461370.49491860183590913No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT334440.3587588642477869No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG329240.3531807453203605No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC316450.3394607181892482No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT280360.30074642740255214No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA230060.24678885393148503No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG229730.24643485792262915No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG226450.24291635213763707No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG198580.21301978011698816No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG160310.1719669702414864No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGGAGA132880.14254239289931206No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAG128390.137725901748515No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG116480.1249498639743518No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA113170.12139917673400923No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGA104790.11240982353942588No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTA103610.11114401962897143No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)