FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11113536
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAT52250.0111.9363861
TGACTAG45900.0111.572831
CGTAACA25250.0109.49533
ACGATAC41850.0108.0850751
CGATTGA21150.0106.254121
ACCGTAA58350.0105.789241
AACGTTG46400.0105.2478561
TCGTACC49450.0104.873971
CACCGAA22150.0104.666058
CGTAAAG23550.0103.335953
CGGACGT34500.0102.927021
TATGCCC37050.0102.647231
ATCCGAA40150.0102.435881
TCCCGAT45300.0101.125451
ACGGGTC55350.0100.0714651
TATTACC57250.0100.0214541
CCGATCA34700.098.350043
GGCTCGA33500.098.259131
ATGCGAT27950.097.154091
TAGACCA46850.097.011071
Produced by FastQC (version 0.11.5)