FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11113536
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC7041306.335787277784497No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC4608604.1468349947307495No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC4360873.9239266422495955No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC2800722.5200980138094664No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC604620.5440392688699618No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT561680.5054017011327447No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG399780.3597234939446815No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT398390.358472766903351No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC384270.3457675396921376No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT347090.31231284084561384No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG274170.2466991603752397No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA270490.2433878830284079No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG270030.2429739733600539No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG243500.21910218313955163No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG196890.17716233609177134No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGGAGA155900.14027938542692445No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAG153270.13791290188829192No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG143340.1289778518736071No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA135600.12201337180173799No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTA124750.11225050245034524No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGA124560.11207953976124252No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG893400.0124.455291
AGTCTCT653400.0111.520751
GTCTCTC716300.0101.758012
TCTCTCA792350.092.042843
TCAGCTG837550.087.03147
CTGATGG2121700.081.084151
CAGCTGG902350.080.933078
GGCTCAA2282050.075.536636
TGATGGC2282700.075.522992
CTCAAAC2295450.075.135448
GCTCAAA2296250.075.1343467
TCAAACA2320400.074.3585749
GATGGCT2327750.073.997973
ATGGCTC2330650.073.8754
TGGCTCA2389150.072.051045
AGCTGGT1041100.070.0570149
TCTCAGC1058050.069.119375
CTCAGCT1123600.065.362546
CTCTCAG1122100.065.161164
TACGGCG12850.043.1447643
Produced by FastQC (version 0.11.5)