FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11252581
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC7117716.32540214551666No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC4668554.148870379160123No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC4412093.920958222829056No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC2832742.5174135604978094No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC617100.5484075164622232No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT566040.5030312601171234No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT402910.35806007528406153No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG402430.3576335064817574No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC390840.3473336472761227No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT348340.30956453457211286No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA275500.2448327188224639No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG274120.24360633351583963No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG271420.24120688400287899No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG244930.21766561822572084No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG198760.17663503155409413No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAG157370.13985235920541253No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGGAGA156900.13943467725315642No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG143290.12733967433782525No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA135800.12068342365187151No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTA127700.11348507511298964No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGA127430.11324513016169356No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG907300.0124.6154251
AGTCTCT662550.0112.418191
GTCTCTC734150.0101.4520342
TCTCTCA804850.092.695723
TCAGCTG850450.087.8182457
CAGCTGG918950.081.444148
CTGATGG2141200.080.882461
GGCTCAA2303850.075.379666
TGATGGC2307200.075.2643052
CTCAAAC2318150.074.9978568
GCTCAAA2317300.074.994637
TCAAACA2344350.074.218379
GATGGCT2348650.073.8283843
ATGGCTC2357600.073.57564
TGGCTCA2409150.071.9713755
AGCTGGT1060050.070.386329
TCTCAGC1086750.068.875965
CTCTCAG1145750.065.329224
CTCAGCT1150450.065.325176
TACGGCG13700.049.401293
Produced by FastQC (version 0.11.5)