FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10895832
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC39250.0108.824761
TGACTAG42100.0108.130361
CGAACAT48700.0107.970861
TCGTACC49950.0107.431951
ACCGTAA56750.0105.601521
ATCCGAA39750.0104.194161
CGTAACA25350.0103.9542853
CGATTGA22350.0103.774591
CGTAAAG22900.0102.813833
AACGTTG43050.0102.063241
CGGACGT31950.0101.225081
TATGCCC37650.0101.205331
TCCCGAT40800.0100.453431
ACGAGCC26100.0100.179491
GACGCGT30600.0100.04151
CACCGAA21600.099.665938
TATTACC53350.099.370181
ATTGGAC41100.099.194431
ATGCGAT25500.099.147011
GGCTCGA31750.099.1403351
Produced by FastQC (version 0.11.5)