FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11005841
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG43850.0109.745351
CGTAACA25800.0108.835743
TCGTACC49350.0108.754781
ACGATAC38850.0108.478611
CACCGAA20950.0107.911158
CGAACAT53450.0106.342921
CGGACGT31500.0105.8887251
CGATTGA20450.0105.68351
TATTACC57800.0104.696161
AACGTTG46800.0104.674841
ATGCGAT25950.0103.550151
ACCGTAA62200.0103.1968841
ATCCGAA40650.0102.257351
CGTAAAG23550.0101.807553
TATGCCC41250.0101.119261
TCCCGAT41300.0100.996831
TCGACCA21500.0100.127518
CCGATCA34050.099.8048253
TGACGGC34450.099.748971
ATTACGA12450.097.7322859
Produced by FastQC (version 0.11.5)