FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11005841
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC7269716.605319847888044No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC4676944.249507148068012No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC4328633.933029743024636No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC2735842.485807309046169No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC631390.5736862816753394No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT585550.5320356708769461No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT410120.3726384925967947No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG392810.356910480534836No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC382920.34792434308291387No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT349040.31714068920312405No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG279990.254401276558511No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA275110.2499672673810207No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG268160.24365243873684891No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG233880.212505341481855No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG190480.17307173527220682No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGGAGA159880.14526831706909085No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAG157870.14344201410869012No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG144920.1316755348364564No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA129080.11728317717837283No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTA128910.11712871374391107No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGA124550.11316718095418606No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG900750.0124.739871
AGTCTCT645650.0111.741151
GTCTCTC711150.0101.487972
TCTCTCA782350.092.416543
TCAGCTG825700.087.319647
CTGATGG2125650.081.08391
CAGCTGG896100.080.620288
TGATGGC2282900.075.623512
GGCTCAA2291300.075.273976
CTCAAAC2300200.074.982038
GCTCAAA2303700.074.9056247
TCAAACA2324550.074.30199
GATGGCT2329050.074.026083
ATGGCTC2336100.073.8088464
TGGCTCA2395900.071.9455955
AGCTGGT1022250.070.523489
TCTCAGC1061900.068.148495
CTCAGCT1114200.065.233966
CTCTCAG1123500.064.48254
TACGGCG14150.045.7948533
Produced by FastQC (version 0.11.5)