FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9996685
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAT48800.0111.2920761
TGACTAG37900.0108.7100141
ACGATAC36400.0108.044821
CGTAACA22700.0107.8402943
ACCGTAA53000.0107.773021
CGTAAAG20250.0107.732233
TCGTACC43400.0107.546921
CACCGAA20600.0104.853818
AACGTTG41700.0104.158321
ATTACGA12050.0103.368759
CGATTGA20650.0103.248651
ATCCGAA36450.0103.153911
TATTACC51700.0102.959541
TCGACCA19850.0100.835748
TAGCCGG30400.0100.699681
ATGCGAT22600.0100.395641
CGGACGT28800.099.54131
CCGATCA30700.099.204193
TAAACCG25000.099.113121
ACGTTAA19900.098.793112
Produced by FastQC (version 0.11.5)