FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9996685
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC6342526.344623242604923No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC4182524.18390696515895No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC3920543.9218400899898316No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC2511812.5126429411349864No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC547030.547211400579292No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT501930.5020964449715081No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT375360.37548447310283356No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG362410.362530178754257No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC341920.34203338406681816No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT305630.30573134994250595No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA254240.2543243085082705No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG252390.2524736950299024No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG244440.24452105873096933No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG215860.21593158131920734No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG174530.1745878758808545No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGGAGA146180.1462284747393761No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAG141330.14137686643122196No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG129710.12975301312385057No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA123290.12333088418810835No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTA114410.11444793949194158No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGA113890.11392776705477865No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG805650.0124.238141
AGTCTCT593950.0111.469591
GTCTCTC658450.0100.528452
TCTCTCA722050.091.812113
TCAGCTG758800.087.2497
CTGATGG1912550.081.0549241
CAGCTGG826950.080.275978
GGCTCAA2050200.075.750046
TGATGGC2055300.075.526912
GCTCAAA2062000.075.324767
CTCAAAC2063550.075.271318
TCAAACA2086050.074.521569
GATGGCT2090850.074.180893
ATGGCTC2098200.073.924484
TGGCTCA2150200.072.123315
AGCTGGT944100.070.1622859
TCTCAGC963000.069.041895
CTCTCAG1016600.065.401684
CTCAGCT1024100.065.2953346
TACGGCG12400.050.518063
Produced by FastQC (version 0.11.5)