FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8958239
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA24700.0114.611251
TACGGGC39500.0112.699771
CCGGTAG30150.0110.378961
TTGAACG28650.0109.3695141
CTTACCG36200.0109.044471
ACGATAC34150.0108.207731
CCTTACG24700.0107.612091
TGACGGC23600.0104.692071
TACGAGA17000.0102.0672454
CATTCGC28200.0101.663631
ACGAGCC24250.0101.5888441
GCCTCTA54500.0101.507091
CGTTAGA23400.0101.277211
TACGGAT26700.0100.900191
TGCGTGG36000.0100.646141
CTACCCA42900.0100.241761
CAATGGA37100.099.942828
TTTAGAC16150.099.41475
TAAACCG21200.099.21531
CACCGAA10750.098.45268
Produced by FastQC (version 0.11.5)