FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7188424
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA19600.0117.607871
TACGGGC31350.0113.0498661
CCGGTAG25400.0110.321051
CCTTACG21550.0107.9686661
CGCTATC20750.0107.271141
ACGATAC26800.0107.245881
CTTACCG30000.0106.371421
TTGAACG22200.0105.780861
ACGAGCC22850.0105.293791
ATTACGA9400.0104.169139
TACGAGA13250.0103.244214
GCCTCTA42250.0102.2979661
TGACGGC20800.0101.818451
TGACTAG18400.0101.396841
CGGACTG20800.0101.125811
TTTAGAC12700.099.7777945
TAGACTA12150.098.961247
CTACCCA34050.098.796661
GATGCGA6450.098.231549
CGGACGT24600.097.8033751
Produced by FastQC (version 0.11.5)