FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8192780
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA21400.0117.473721
TACGGGC37550.0116.633321
CCGGTAG30350.0112.9736941
ACGATAC27550.0109.813761
TTGAACG26250.0109.763951
TGACGGC22400.0109.656751
CCTTACG20150.0107.959511
CTTACCG31550.0106.1653061
GACCAAT29200.0105.0886461
ACGAGCC25450.0104.157141
CAATGGA35850.0103.2270668
TACGAGA14200.0102.4194954
CGCTATC22800.0101.414311
CTACCCA37650.0101.2091451
TGCGTGG33750.0101.165771
GCCTCTA48400.0101.0539861
CGCTTCA27900.0100.9489061
CGGACTG23200.0100.907721
TACGGAT23500.099.926061
CGGACGT28600.099.7374341
Produced by FastQC (version 0.11.5)