FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13352980
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA29000.0112.510031
TAAACCG37300.0110.0669861
CCGGTAG53400.0106.960391
CCGAACG33100.0106.80189
TACGGGC50300.0104.674391
TTAGGCA45150.0102.89471
ACGATAC52050.0101.293461
CACCGAA25400.0100.628228
TCCCGAT46500.0100.062331
CGTCTAC42600.099.923791
CCTTACG36300.099.6066061
TACTTAC65400.099.1187441
CGCTATC34250.097.787511
TACGGTA45700.096.7705461
TGACGGC34100.096.739121
TACCTTA51000.096.599961
TACACGA37700.096.4808351
AGCGATA21650.096.109228
TTGAACG33750.096.035051
GCGCGAT38350.095.5968251
Produced by FastQC (version 0.11.5)