FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13352980
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC5036553.771854672140601No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC3483942.6091104757140355No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC2389461.7894582332932423No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC1620321.213451978509666No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC534950.4006221832130356No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT464700.3480122040173804No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC258790.1938069254952827No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT220490.16512418950676178No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG210260.1574629783014728No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG192070.14384055094817785No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT179710.13458419019574658No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG134540.10075653524531601No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGGATG133610.10006006149938067No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG672050.0114.14881
GTCTCTC609700.0100.721062
AGTCTCT620800.098.874111
TCTCTCA640000.096.065993
TCAGCTG665600.092.3254557
CAGCTGG693050.088.9481668
AGCTGGT714300.086.039949
TCTCAGC852050.072.293225
CTGATGG1566550.071.206711
GGCTCAA1599550.069.775756
CTCAGCT890750.069.491826
GCTCAAA1613800.069.162277
CTCAAAC1617600.069.021288
CTCTCAG898550.068.59214
TCAAACA1631100.068.480919
ATGGCTC1668400.066.935154
GATGGCT1699350.065.652513
TGGCTCA1711800.065.082485
TGATGGC1875000.059.7767452
TGATACG19150.050.7583663
Produced by FastQC (version 0.11.5)