FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10544951
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA22600.0115.3727951
CCGAACG26200.0105.524689
CCGGTAG44500.0105.048051
TAAACCG29650.0104.945141
ACGATAC39250.0104.785151
CGCTATC24950.0104.217331
TCCCGAT38000.0103.303771
CACCGAA21600.0101.66478
TACGGGC38250.0101.498731
TACACGA29100.0100.988161
CCTTACG27550.0100.395191
TGACGGC27350.0100.339271
TTAGGCA37400.0100.146331
AGTACGA29950.098.321978
TACTTAC51350.098.188551
GCGCAAC23350.096.551871
TACCTTA39000.096.4071351
CGTCTAC32650.096.405431
ATTCTAC60100.095.877961
TACGGTA40000.095.437521
Produced by FastQC (version 0.11.5)