FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10648143
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC4007733.7637830370985816No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC2779712.6105115229951363No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC1895521.7801413823987902No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC1284861.2066517138246546No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC435370.40886941507077806No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT370500.3479479943122477No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC204380.19193957105947956No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT171590.161145469214679No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG167670.15746407613045768No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG152140.14287937342689708No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT142760.1340703256896531No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG106800.1002991789272552No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG534700.0114.258431
GTCTCTC482450.0100.6270752
AGTCTCT483850.0100.396871
TCTCTCA500550.097.193033
TCAGCTG525300.092.353267
CAGCTGG547750.088.673248
AGCTGGT565850.085.696859
TCTCAGC675250.072.068675
CTGATGG1257200.071.578221
GGCTCAA1280550.070.169236
GCTCAAA1290900.069.673567
CTCAAAC1290950.069.626248
TCAAACA1302700.069.119839
CTCAGCT707900.068.927776
CTCTCAG710350.068.659634
ATGGCTC1336850.067.241054
GATGGCT1359550.066.160713
TGGCTCA1366600.065.6982355
TGATGGC1495650.060.3535162
TGATACG13750.057.599493
Produced by FastQC (version 0.11.5)